The rubber kernel is rich in nutrients. But its toxicity due to hydrocyanic acid is a problem for its use in food. This bitter almond contains a high concentration of cyanide (2712.4 mg/kg). Its extracts are therefore declared not to comply with the recommendations of the food standards. However, the rubber plantations in Côte d'Ivoire provide a large quantity of seeds, and only 10% of these seeds are used to make plants. The remaining 90% is left in the plantations, which represents an annual production of about 75,000 to 100,000 tons of rubber seeds to be used in Ivorian rubber farming. It is in this context that the cyanogenetic study of the kernel was conducted. The evaluation of the total and free cyanide content of the extracts during operations such as: solar drying of the seeds and kernels in an oven, optimization of the biochemical hydrolysis of cyanoglycosides and roasting of the crushed material, made it possible to develop the present process for detoxifying the extracts from the kernel, particularly the oil and the cake. The residual hydrocyanic acid content decreased from 2712.4mg/kg to 0.38mg/kg (<35mg/Kg), as recommended by the standards for cyanogenic almonds. The rubber tree oil produced had an essential fatty acid profile of 30.9% omega 6 and 6.2% omega 3. And the study of the acute and sub-acute toxicity of the oil produced was carried out with the aim of valorising the rubber seeds in the food and cosmetic field. The absence of deaths and clinical signs observed would indicate that the oil produced by this process is not toxic at the high dose of 2000mg/kg. Pc and at the dose of 5000mg/kg. Pc. Evaluation of chronic administration of the extract on relative organ weights and determination of biochemical parameters such as urea; creatine (CREA); alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and alkaline phosphatase (ALP) activity reveal that the oil does not contain toxic substances that would contribute to disrupting the integrity of liver tissue. The evaluation of the chemical and biological properties reveals that, after being detoxified into cyanides, rubber tree oil can be used in food or in cosmetics.
| Published in | American Journal of BioScience (Volume 10, Issue 6) |
| DOI | 10.11648/j.ajbio.20221006.16 |
| Page(s) | 220-229 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Detoxification, Cyanogenic Glycosides, Total Cyanides, Acute Toxicity, Subacute Toxicity, Rubber Oil
| [1] | Kouakou N’G. D. V., Angbo-Kouakou C. E. M., Kone G. A., Kouame K. B., Yéboué F. de P., Kouba M. (2018). Enhancement of rubber kernel and cashew nut cakes in the diet of postweaning and growing pigs. Rev. Elev. Med. Vet. Pays Trop, 71 (1-2): 81-85, doi: 10.19182/remvt.31256. |
| [2] | Eka H. D., Tajul Aris Y., Wan Nadiah W. A. (2010). Potential use of Malaysian rubber (Hevea brasiliensis) seed as food, feed and biofuel. Int. Food Res. J., 17: 527-534. |
| [3] | M. Bisson, J. Bureau, S. Deny, J. P. Lefevre, K. Tack (2011). Cyanides and derivatives. INERIS - Toxicological and environmental data sheets on chemical substances p 17-40. |
| [4] | P. Campo, D. Jargot, B. La Rocca, J. Passeron, F. Pillière and S. Robert. Robert (2018). Hydrogen cyanide and aqueous solutions. February 2018 edition, P 1-9. [Online] Available: http:// WWW.inrs.fr/Fichetox. |
| [5] | Draft screening assessment, Cyanides. (2018). Environment and Climate Change Canada, Health Canada February 2018, p 2-83. |
| [6] | Okoma Muriel, Kouadio Eugène, Ban Koffi Louis, Amoakon Evelyne (2018). Hevea seeds, a by-product to be valorized in Côte d'Ivoire. Le CNRA en 2018, p 14-15. |
| [7] | Kouakou N’G. D V, Coulibaly. S B M, Angbo-Kouakou. C E M, Ahongo. Y D, Assidjo. N E, Kouba M. (2019). Rabbit meat (Oryctolagus cuniculus L.) enriched in omega 3 with a feed containing euphorbia (Euphorbia heterophylla L.). Rev. Elev. Med. Vet. Pays Trop., 72 (3): 107-113. Doi: 10.19182/remvt.31779. |
| [8] | Ariadna Gomez-Vilarrubla, Berta Mas-Parés, Marta Díaz, Sílvia Xargay-Torrent, Gemma Carreras-Badosa, Mariona Jové, Meritxell Martin-Gari, Alexandra Bonmatí-Santané, Francis de Zegher, Lourdes Ibañez, Abel Lopez-Bermejób, Judith Bassols. (2021). Fatty acids in the placenta of appropiate- versus small-for-gestational-ageinfants at term birth. Journal homepage: http://www.elsevier.com/locate/placenta. Placenta 109 (2021) 4–10. |
| [9] | Nisha S. Wadhwani, Kamini D. Dangat, Asmita A. Jashi, Sadhana R. Joshi. (2013). Maternal micronutrients and omega 3 fatty acids affect placental fatty acid desaturases and transport proteins in wistar rats. Prostaglandins, leukotrienes and Essential Fatty acids 88 (2013) 235-242. |
| [10] | (Friedman, M. (2006). Potato Glycoalkaloids and Metabolites: Roles in the Plant and in the Diet. Journal of Agricultural and Food Chemistry, 54 (23), 8655–8681. https://doi.org/10.1021/jf061471 |
| [11] | Yulvianti. M, Zidorn. C. (2021). Chemical Diversity of Plant Cyanogenic Glycosides: An Overview of Reported Natural Products. Molecules 2021, 26, 719. https://doi.org/10.3390/molecules26030719 |
| [12] | Atchibri. L O, Atcho. O, Kouakou. B, Keli. J. (2008). Hevea seed depleted in hydrocyanic acid by triple drying method offers a suitable meal for laying hen consumption. African Journal of Animal Health and Production, 6 (3-4), 195-198. |
| [13] | Berriozabalgoitia. A, Ruiz de Gordoa. J C, Amores. G, & Virto. M. (2022). Dietary fatty acid metabolism: New insights in Potential hazards of feeding albino rats on diet containing repeatedly boiled cooking oil: Clinicopathological and Toxicological studies to the similarities of lipid metabolism in humans and hamsters. Food Chemistry: Molecular Sciences, 4, 100060. https://doi.org/10.1016/j.fochms.2021.100060 |
| [14] | CEAEQ. (2016). Determination of cyanides: Automated colorimetric method with isonicotinic acid and barbituric acid - manual distillation. https://www.ceaeq.gouv.qc.ca/methodes/pdf/MA300CN12. |
| [15] | K. Oudjehani, G J. Zagury, L. Deschênes. (2002). Natural attenuation potential of cyanide via microbial activity in mine tailings. Original paper, Appl Mirobiol Biotechnol (2002) 58: 409–415. DOI 10.1007/s00253-001-0887-2. |
| [16] | N'zué. B, Zouhouri. P G, & Sangaré. A. (2004). Agronomic performances of some cassava varieties (Manihot esculenta Crantz) in three agro- climatic zones of Cote D'Ivoire. Agronomie Africaine, 16 (2), 1-7. |
| [17] | Augustin Koffi YAO, Djary Michel KOFFI, Sika Hortense BLEI, Zaouli Bi IRIÉ and Sébastien Lamine NIAMKE (2015). Biochemical and organoleptic properties of three traditional Ivorian dishes (attiéké, placali, attoukpou) based on native cassava granules. J. Biol. Chem. Sci. 9 (3): 1341-1353. Available online at http://ajol.info/index.php/ijbcs. |
| [18] | Yéboué. K H, Amoikon. K E, Kouamé. K G, & Kati-Coulibaly S. (2017). Nutritional value and organoleptic properties of attiéké, attoukpou and placali, three cassava-based dishes commonly consumed in Côte d'Ivoire. Journal of Applied Biosciences, 113 (1), 11184. https://doi.org/10.4314/jab.v113i1. |
| [19] | Trincone A. (2015). Uncommon Glycosidases for the Enzymatic Preparation of Glycosides. Biomolecules, 5 (4), 2160–2183. https://doi.org/10.3390/biom5042160 |
| [20] | Zuzana Mészáros, Pavlína Nekvasilová, Pavla Bojarová, Vladimír Kren, Kristýna Slámová. (2021). Reprint of: Advanced glycosidases as ingenious biosynthetic instruments. Biotechnology Advances 51 (2021) 107820. https://doi.org/10.1016/j.biotechadv.2021.107733 |
| [21] | Liebig-Denigès. (1948). Research and determination of the hydrocyanic acid content in bitter almonds. Journal Officiel published 27 February and 5-6 April 1948. Brunel, Traité pratique de Chimie Végétale, Tome 5, p 540-543. |
| [22] | Leibig-Denigès. (1971). Determination of hydrocyanic acid. Meled. Agricultural College: Wageningen 71, 13p. |
| [23] | M. Rezaul Haque, J. Howard Bradbury. (2002). Total cyanide determination of plants and foods using the picrate and acid hydrolysis methods. Food chemistry 77 (2002) 104-114. |
| [24] | M. Rezaul Haque, J. Howard Bradbury (2004). Preparation of linamarin from cassava leaves for use in a cassava cyanide it. Food chemistry 85 (2004) 27-29. https://doi.org/10.1016/j.foodchem.2003.06.001 |
| [25] | Norme internationale ISO (2164). Pulses-Determination of glycosidic hydrocyanic acid. Première édition-1975-09-15. |
| [26] | Puskarczyk. E. (2006). Current toxicokinetic, toxicodynamic and therapeutic aspects of acute cyanide ion intoxication [PhD Thesis]. UHP-Université Henri Poincaré. |
| [27] | OECD, (2001). OECD Test Guideline for Chemicals No. 407 and 423: Acute Oral Toxicity-Acute Toxicity Class Method; 14 p. |
| [28] | Bürger. C, Fischer. D R, Cordenunzzi. D A, Batschauer de Borba. A P, Filho. V C, & Soares dos Santos. R. (2005). Acute and subacute toxicity of the hydroalcoholic extract from Wedelia paludosa (Acmelabrasilinsis) (Asteraceae) in mice. Journal of Pharmaceutical Sciences, 8 (2): 370-373. |
| [29] | Badr El Said El-bialy, Nahed Thabet Saleh, Reham Mohamed Abou-Elkhair. (2015). Potential hazards of feeding albino rats on diet containing repeatedly boiled cooking oil: Clinicopathological and Toxicological studies. International Journal of Advanced Research (2015), Volume 3, Issue 3, 134-147. |
| [30] | Frédéric Lappostolle, Yves Lambert (2020). Fire smoke poisoning. Medecine de catastrophe-Urgences collectives (2020); 4 (2): 133-137. |
| [31] | Juliette Bloch (2018). Bitter apricot kernels: to be consumed in great moderation. Vigil 'Anses, Anses, 2018, pp. 4-5. Anses-01828406. |
| [32] | World Health Organization. (2013). CODEX ALIMENTARUIS 2013 on the manufacture of cyanogenic foods.pdf |
| [33] | EFSA (European Food Safety Authority), (2018). Report for 2016 on the results from the monitoring of veterinary medicinal product residues and other substances in live animals and animal products. EFSA supporting publication 2018: EN-1358. 75 pp. doi: 10.2903/sp.efsa.2018.EN-1358. |
| [34] | Amani. G, Nindjin. C, N'zué. B, Tschannen. A, & Aka. D. (2007). Potential for cassava (Manihot esculenta crantz) processing in West Africa. Proceedings of the 1st International Workshop, p358. |
| [35] | Gningnini Alain Kone (2019). Effect of incorporating rubber tree (Hevea brasiliensis) seed cake or cashew nut (Anacardium occidentale) cake on the performance of guinea fowl (Numida meleagris) in Côte d'Ivoire. Science of animal productions. Agrocampus Ouest, French. NNT: 2019NSARC143. |
| [36] | Yuan Si, Xiao-Song Sha, Lei-Ling Shi, Hong-Yan Wei, Yue-Xian Jin, Guo-Xu Ma, Jing Zhang. (2022). Review on Pregnane Glycosides and Their Biological Activities. Phytochemistry Letters 47 (2022) 1–17. https://doi.org/10.1016/j.phytol.2021.10.007 |
| [37] | Cacho. J. (2012). New acquisitions on the hydrolysis of glycosidic compounds in wine. Aromes Du Vin Aromas Del Vino, 15. Universidad Zaragoza, pedro cerbuna 12, 5009 ZARAGOZA, Espagne. E-mail: jcacho@unizar.es |
| [38] | Silitonga. A S, Masjuki. H H, Ong. H C, Yusaf. T, Kusumo. F, & Mahlia. T M I. (2016). Synthesis and optimization of Hevea brasiliensis and Ricinus communis as feedstock for biodiesel production: A comparative study. Industrial Crops and Products, 85, 274–286. https://doi.org/10.1016/j.indcrop.2016.03.017 |
| [39] | Antonio Morata, Carmen González, Wendu Tesfaye, Iris Loira and Jose A. Suárez-Lepe. (2019). Maceration and Fermentation: New Technologies to Increase Extraction. DOI: https://doi.org/10.1016/B978-0-12-814399-5.00003-7 |
| [40] | Nathalie. A, Hélène. E, Florian. G, François. P, Amandine. F, Anne. B, Françoise. S, Virginie. S. (2016). Cyanides concentrations in post-morten samples: difficulties of interpretation. Toxicologie analytique & clinique (2016) 28, 164-169. https://dx. doi.org/10.1016/j.toxac.2016.03.001 |
| [41] | Sirwal. I A, Banday. K A, Reshi. A R, Bhat. M A, Wani. M M. (2004). Estimation of Glomerular Filteration Rate (GFR). JK Science. (2004); 6: 121–1. |
| [42] | Kone. G. Alain, Kouakou. N'. G. D. Vincent, Angbo-kouakou. C. E. Monique, Kouamé. K. Bertin, Yeboue. F. de Paul, Kouba. M. (2016). Preliminary Study of The Valorization of Rubber, Cashew and Jatropha Oil Cakes in Pigs During Gestation and Lactation. European Scientific Journal, Vol 12, p1-12. doi: 10.19044/esj.2016.v12n30p11 URL: http://dx.doi.org/10.19044/esj.2016.v12n30p11. |
| [43] | Combes S., Auvergne A., Darche B. & Lebas F. (2001). Evolution with age of the mechanical resistance of bones in rabbits. 9th Journées de la Recherche Cunicole, Paris Nov, 15-18. |
| [44] | Raza M., Al-Shabanah O. A., El-Hadiyah T. M. & Al-Majed A. A. (2002). Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Scientia Pharmaceutica, 70: 135–145. |
| [45] | Peirs C. (2005). Contribution to the phytochemical study of Galega officinalis L. (Fabaceae). Thesis presented in order to obtain the degree of Doctor of Pharmacognosy from the Institut National Polytechnique de Toulouse. 230p. |
| [46] | Débora. L R P, Maria. S S C, Sonia. M F F, Marilene. O R B, & Antonio. C R B. (2013). Acute and sub-chronic pre-clinical toxicological study of Averrhoa carambola L. (Oxalidaceae). African Journal of Biotechnology, 12 (40): 5917-5925. |
| [47] | Sacher. R A, & McPherson R. A. (1991). Widmann’s Clinical Interpretation of Laboratory Test. Pennsylvania, USA, pp. 416–443. |
| [48] | Pocock G. & Richards C. D. (2006). Human Physiology. The basis of Medicine 225-246. |
| [49] | James S. & Mitchel G. (2006). Physiology and disorder of water electrolytes and acid base metabolism. In Carl A. B., Edward R, David E. editors. Tietz Textbook of clinical chemistry and molecular diagnostics, 4th edition. NewDelhi, Elsevier Inc: pp 1747-1776. |
| [50] | Mohamed Saleh AbdelFattah, Sherif EA Badr, Eid M Khalil1 and Ahmed Salah El said. (2020). Feed efficiency, some blood parameters and In- vitro chemoprevention of prickly pear (Opuntia ficus indica L.) seeds oil growing in Egypt. Biological Sciences and Pharmaceutical Research Vol. 8 (1). https://doi.org/10.15739/ibspr.20.002i |
APA Style
Bamba Soualiho, Jean-Claude N'Guessan Yao, Kouadio Kouakou John, Kanate Losseyni, Adima Amissa Augustin. (2022). Evaluation of the Chemical and Biological Properties of Oil Extracted from Detoxified Rubber Tree (Hevea Brasiliensis) Kernels. American Journal of BioScience, 10(6), 220-229. https://doi.org/10.11648/j.ajbio.20221006.16
ACS Style
Bamba Soualiho; Jean-Claude N'Guessan Yao; Kouadio Kouakou John; Kanate Losseyni; Adima Amissa Augustin. Evaluation of the Chemical and Biological Properties of Oil Extracted from Detoxified Rubber Tree (Hevea Brasiliensis) Kernels. Am. J. BioScience 2022, 10(6), 220-229. doi: 10.11648/j.ajbio.20221006.16
AMA Style
Bamba Soualiho, Jean-Claude N'Guessan Yao, Kouadio Kouakou John, Kanate Losseyni, Adima Amissa Augustin. Evaluation of the Chemical and Biological Properties of Oil Extracted from Detoxified Rubber Tree (Hevea Brasiliensis) Kernels. Am J BioScience. 2022;10(6):220-229. doi: 10.11648/j.ajbio.20221006.16
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author = {Bamba Soualiho and Jean-Claude N'Guessan Yao and Kouadio Kouakou John and Kanate Losseyni and Adima Amissa Augustin},
title = {Evaluation of the Chemical and Biological Properties of Oil Extracted from Detoxified Rubber Tree (Hevea Brasiliensis) Kernels},
journal = {American Journal of BioScience},
volume = {10},
number = {6},
pages = {220-229},
doi = {10.11648/j.ajbio.20221006.16},
url = {https://doi.org/10.11648/j.ajbio.20221006.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221006.16},
abstract = {The rubber kernel is rich in nutrients. But its toxicity due to hydrocyanic acid is a problem for its use in food. This bitter almond contains a high concentration of cyanide (2712.4 mg/kg). Its extracts are therefore declared not to comply with the recommendations of the food standards. However, the rubber plantations in Côte d'Ivoire provide a large quantity of seeds, and only 10% of these seeds are used to make plants. The remaining 90% is left in the plantations, which represents an annual production of about 75,000 to 100,000 tons of rubber seeds to be used in Ivorian rubber farming. It is in this context that the cyanogenetic study of the kernel was conducted. The evaluation of the total and free cyanide content of the extracts during operations such as: solar drying of the seeds and kernels in an oven, optimization of the biochemical hydrolysis of cyanoglycosides and roasting of the crushed material, made it possible to develop the present process for detoxifying the extracts from the kernel, particularly the oil and the cake. The residual hydrocyanic acid content decreased from 2712.4mg/kg to 0.38mg/kg (<35mg/Kg), as recommended by the standards for cyanogenic almonds. The rubber tree oil produced had an essential fatty acid profile of 30.9% omega 6 and 6.2% omega 3. And the study of the acute and sub-acute toxicity of the oil produced was carried out with the aim of valorising the rubber seeds in the food and cosmetic field. The absence of deaths and clinical signs observed would indicate that the oil produced by this process is not toxic at the high dose of 2000mg/kg. Pc and at the dose of 5000mg/kg. Pc. Evaluation of chronic administration of the extract on relative organ weights and determination of biochemical parameters such as urea; creatine (CREA); alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and alkaline phosphatase (ALP) activity reveal that the oil does not contain toxic substances that would contribute to disrupting the integrity of liver tissue. The evaluation of the chemical and biological properties reveals that, after being detoxified into cyanides, rubber tree oil can be used in food or in cosmetics.},
year = {2022}
}
TY - JOUR T1 - Evaluation of the Chemical and Biological Properties of Oil Extracted from Detoxified Rubber Tree (Hevea Brasiliensis) Kernels AU - Bamba Soualiho AU - Jean-Claude N'Guessan Yao AU - Kouadio Kouakou John AU - Kanate Losseyni AU - Adima Amissa Augustin Y1 - 2022/12/23 PY - 2022 N1 - https://doi.org/10.11648/j.ajbio.20221006.16 DO - 10.11648/j.ajbio.20221006.16 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 220 EP - 229 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20221006.16 AB - The rubber kernel is rich in nutrients. But its toxicity due to hydrocyanic acid is a problem for its use in food. This bitter almond contains a high concentration of cyanide (2712.4 mg/kg). Its extracts are therefore declared not to comply with the recommendations of the food standards. However, the rubber plantations in Côte d'Ivoire provide a large quantity of seeds, and only 10% of these seeds are used to make plants. The remaining 90% is left in the plantations, which represents an annual production of about 75,000 to 100,000 tons of rubber seeds to be used in Ivorian rubber farming. It is in this context that the cyanogenetic study of the kernel was conducted. The evaluation of the total and free cyanide content of the extracts during operations such as: solar drying of the seeds and kernels in an oven, optimization of the biochemical hydrolysis of cyanoglycosides and roasting of the crushed material, made it possible to develop the present process for detoxifying the extracts from the kernel, particularly the oil and the cake. The residual hydrocyanic acid content decreased from 2712.4mg/kg to 0.38mg/kg (<35mg/Kg), as recommended by the standards for cyanogenic almonds. The rubber tree oil produced had an essential fatty acid profile of 30.9% omega 6 and 6.2% omega 3. And the study of the acute and sub-acute toxicity of the oil produced was carried out with the aim of valorising the rubber seeds in the food and cosmetic field. The absence of deaths and clinical signs observed would indicate that the oil produced by this process is not toxic at the high dose of 2000mg/kg. Pc and at the dose of 5000mg/kg. Pc. Evaluation of chronic administration of the extract on relative organ weights and determination of biochemical parameters such as urea; creatine (CREA); alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and alkaline phosphatase (ALP) activity reveal that the oil does not contain toxic substances that would contribute to disrupting the integrity of liver tissue. The evaluation of the chemical and biological properties reveals that, after being detoxified into cyanides, rubber tree oil can be used in food or in cosmetics. VL - 10 IS - 6 ER -
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