Elucidation of the in vitro antitumor effects of Pleurotus ostreatus polysaccharides is very important for their future clinical application. In this paper, we use Sarcoma-180 (S180) cells to test the antitumor activity of the mushroom polysaccharides. The S180 cells in freshly prepared mouse ascites were directly treated by water-soluble polysaccharides from mycelium of Pleurotus ostreatus and immediately detected by MTT [3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide] method. The results can be directly visualized with naked eyes through the color changes. The in vitro antitumor activity of P. ostreatus polysaccharides on S180 cells are dose-dependently and time-dependently. P. ostreatus strains p11, p23, P44, p105 and p176 showed significant inhibition to S180 cell activity under high polysaccharides concentration (1000μg/ml ~ 1250μg/ml), and there are non-significant effects under the middle concentration (750μg/ml). On the contrary, the S180 cell activity were significantly enhanced under low concentration (250μg/mL-500μg/ml). The effects of polysaccharides generally increased during 4-8 treat hours, but remained stable more than 7 hours. However, the strains P23, P44 and P105 enhanced the S180 cell activity for short time processing (4-5h), but inhibited the cell activity for long time processing (6-8h). Among the tested mushroom strains, P44 exhibited the highest inhibition rate (68.4%) under high concentration of 1250µg/ml and treat time of 7-8 hours, P23 had the highest enhancement (119.5%) under low concentration of 250µg/ml and treat time of 8 hours. The antitumor activity of P. ostreatus polysaccharides was dependent on its concentration and treat time, indicating the complicated antitumor mechanism of mushroom polysaccharides and the strict study should be conducted before their clinical application.
| Published in | American Journal of BioScience (Volume 10, Issue 2) |
| DOI | 10.11648/j.ajbio.20221002.16 |
| Page(s) | 75-80 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Pleurotus ostreatus, Polysaccharides, MTT, S180 Cell, Antitumor Activity
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APA Style
Zhang Shuhong, Zhang Yunfeng, Gao Fengju, Fan Yongshan. (2022). Dose-Dependent and Time-Dependent Antitumor Activity of Pleurotus ostreatus Polysaccharides in Vitro. American Journal of BioScience, 10(2), 75-80. https://doi.org/10.11648/j.ajbio.20221002.16
ACS Style
Zhang Shuhong; Zhang Yunfeng; Gao Fengju; Fan Yongshan. Dose-Dependent and Time-Dependent Antitumor Activity of Pleurotus ostreatus Polysaccharides in Vitro. Am. J. BioScience 2022, 10(2), 75-80. doi: 10.11648/j.ajbio.20221002.16
AMA Style
Zhang Shuhong, Zhang Yunfeng, Gao Fengju, Fan Yongshan. Dose-Dependent and Time-Dependent Antitumor Activity of Pleurotus ostreatus Polysaccharides in Vitro. Am J BioScience. 2022;10(2):75-80. doi: 10.11648/j.ajbio.20221002.16
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Download@article{10.11648/j.ajbio.20221002.16,
author = {Zhang Shuhong and Zhang Yunfeng and Gao Fengju and Fan Yongshan},
title = {Dose-Dependent and Time-Dependent Antitumor Activity of Pleurotus ostreatus Polysaccharides in Vitro},
journal = {American Journal of BioScience},
volume = {10},
number = {2},
pages = {75-80},
doi = {10.11648/j.ajbio.20221002.16},
url = {https://doi.org/10.11648/j.ajbio.20221002.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20221002.16},
abstract = {Elucidation of the in vitro antitumor effects of Pleurotus ostreatus polysaccharides is very important for their future clinical application. In this paper, we use Sarcoma-180 (S180) cells to test the antitumor activity of the mushroom polysaccharides. The S180 cells in freshly prepared mouse ascites were directly treated by water-soluble polysaccharides from mycelium of Pleurotus ostreatus and immediately detected by MTT [3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide] method. The results can be directly visualized with naked eyes through the color changes. The in vitro antitumor activity of P. ostreatus polysaccharides on S180 cells are dose-dependently and time-dependently. P. ostreatus strains p11, p23, P44, p105 and p176 showed significant inhibition to S180 cell activity under high polysaccharides concentration (1000μg/ml ~ 1250μg/ml), and there are non-significant effects under the middle concentration (750μg/ml). On the contrary, the S180 cell activity were significantly enhanced under low concentration (250μg/mL-500μg/ml). The effects of polysaccharides generally increased during 4-8 treat hours, but remained stable more than 7 hours. However, the strains P23, P44 and P105 enhanced the S180 cell activity for short time processing (4-5h), but inhibited the cell activity for long time processing (6-8h). Among the tested mushroom strains, P44 exhibited the highest inhibition rate (68.4%) under high concentration of 1250µg/ml and treat time of 7-8 hours, P23 had the highest enhancement (119.5%) under low concentration of 250µg/ml and treat time of 8 hours. The antitumor activity of P. ostreatus polysaccharides was dependent on its concentration and treat time, indicating the complicated antitumor mechanism of mushroom polysaccharides and the strict study should be conducted before their clinical application.},
year = {2022}
}
TY - JOUR T1 - Dose-Dependent and Time-Dependent Antitumor Activity of Pleurotus ostreatus Polysaccharides in Vitro AU - Zhang Shuhong AU - Zhang Yunfeng AU - Gao Fengju AU - Fan Yongshan Y1 - 2022/04/20 PY - 2022 N1 - https://doi.org/10.11648/j.ajbio.20221002.16 DO - 10.11648/j.ajbio.20221002.16 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 75 EP - 80 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20221002.16 AB - Elucidation of the in vitro antitumor effects of Pleurotus ostreatus polysaccharides is very important for their future clinical application. In this paper, we use Sarcoma-180 (S180) cells to test the antitumor activity of the mushroom polysaccharides. The S180 cells in freshly prepared mouse ascites were directly treated by water-soluble polysaccharides from mycelium of Pleurotus ostreatus and immediately detected by MTT [3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide] method. The results can be directly visualized with naked eyes through the color changes. The in vitro antitumor activity of P. ostreatus polysaccharides on S180 cells are dose-dependently and time-dependently. P. ostreatus strains p11, p23, P44, p105 and p176 showed significant inhibition to S180 cell activity under high polysaccharides concentration (1000μg/ml ~ 1250μg/ml), and there are non-significant effects under the middle concentration (750μg/ml). On the contrary, the S180 cell activity were significantly enhanced under low concentration (250μg/mL-500μg/ml). The effects of polysaccharides generally increased during 4-8 treat hours, but remained stable more than 7 hours. However, the strains P23, P44 and P105 enhanced the S180 cell activity for short time processing (4-5h), but inhibited the cell activity for long time processing (6-8h). Among the tested mushroom strains, P44 exhibited the highest inhibition rate (68.4%) under high concentration of 1250µg/ml and treat time of 7-8 hours, P23 had the highest enhancement (119.5%) under low concentration of 250µg/ml and treat time of 8 hours. The antitumor activity of P. ostreatus polysaccharides was dependent on its concentration and treat time, indicating the complicated antitumor mechanism of mushroom polysaccharides and the strict study should be conducted before their clinical application. VL - 10 IS - 2 ER -
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