Haemonchus contortus (H. contortus) parasite causes various functional derangements in the host body. This experimental study was established to appraise the remedial effect of Balanites aegyptiaca fruit’s ethanolic extract (BAF-EE) on experimentally induced haemonchosis in goats. Blood serum biochemical parameters and pathological changes were perused. Twelve male goats were randomly divided into 4 equal groups, G1 (infected-untreated), G2 (infected- BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Experimental infection was conducted with a single oral dose of 10x3 infective third larval stage (L3) of H. contortus at 0-time, whereas treatment with BAF-EE and albendazole was given at a single oral dose of 9g and 5mg/kg body wieght respectively in the 5th week post infection (PI). Blood samples were drawn on zero time, 3rd week PI and then biweekly till the 9th week for serum biochemical analyses. At the end of the experiment, all animals were slaughtered to estimate histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum protein (TP), albumin, albumin/globulin (A/G) ratio, total antioxidant capacity (TAC) and calcium (Ca) levels compared to uninfected goats. Significant elevation was observed in values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (γGT), cholesterol, urea and creatinine of infected animals, while no significant differences were recorded in levels of inorganic phosphorus (P), potassium (K), sodium (Na), chloride (Cl) and total iron binding capacity (TIBC). Histopathological studies conducted on abomasa of infected-untreated animals revealed inflammation, necrosis, petechial hemorrhages and intense cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Histopathological alterations in tissues of infected – treated animals were lower in intensity than those of infected-untreated goats. Results of the present experiment demonstrated that BAF-EE has an anti-parasitic and hepatoprotective effects as well as it improves serum biochemical alterations and oxidative stress in goats infected with H. contortus parasite. The present findings nominate the use of this plant as a novel safe natural anthelmintic agent.
| Published in | American Journal of BioScience (Volume 9, Issue 3) |
| DOI | 10.11648/j.ajbio.20210903.14 |
| Page(s) | 95-104 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Haemonchosis, Goats, Blood Serum Biochemistry, Pathology, Balanites aegyptiaca
| [1] | Adam NAM (2006) Effect of piper Abyssinica and Jatropha curcas against experimental Haemochus contortus infection in desert goats. MVSc Thesis University of Khartoum Sudan. |
| [2] | Ahmad A, Anwar-ul-Hassan C, Anwar-ul-Haq A and Majeed MA (1990) Serum proteinogram of lambs experimentally induced Haemonchus contortus infection. Veterinarsk. 60 (4): 195-200. |
| [3] | Albadawi ROE (2010) In vivo and in vitro anthelmintic activity of Balanites aegyptiaca and Artemisia herba Alba on Haemonchus contortus of sheep. PhD Thesis Faculty of Veterinary Science University of Khartoum. |
| [4] | Al-jebory ZE, Al-Khayat DA (2012) Effect of Haemonchus contortus Infection on Physiological and Immunological Characters in Local Awassi Sheep and Black Iraqi Goats. Journal of Advanced Biomedical & Pathobiology Research 2 (2): 71–80. |
| [5] | Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC (1978) Enzymatic determination of total serum cholesterol. Clinical chemistry 20: 470 – 475. |
| [6] | Al-Malki JS (2017) Epidemylogical and histopathological Studies of Haemonchus contortus among goats in Taif KSA. International Journal of Pharmaceutical Research & Allied Sciences 6 (1): 161–174. |
| [7] | Balic A, Bowles VM, Meeusen ENT (2000) Cellular profiles in the abomasal mucosa and lymph node during primary infection with Haemonchus contortus in sheep. Veterinary Immunology and Immunopathology 75 (1–2): 109–120. |
| [8] | Baragob AEA, AlMalki WH, Shahid I et al. (2014) The hypoglycemic effect of the aqueous extract of the fruits of Balanites aegyptiaca in Alloxan-induced diabetic rats. Pharmacognosy Res 6 (1): 1-5. |
| [9] | Batra S, Srivastava JK, Gupta S, Katiyar JV, Srivastava VML (1993) Role of reactive oxygen species in expulsion of Nippostrongylus brasiliensis from rat. Parasitol 106: 185-192. |
| [10] | Ben-Smith A, Lammas DA, Behnke JM (2002) Effect of oxygen radicals and differential expression of catalase and superoxide dismutase in adult Heligmosomoides polygyrus during primary infections in mice with differing response phenotypes. Parasite Immunol 24: 119–129. |
| [11] | Blood DC, Radostits OM (1989) Veterinary Medicine, A text book of the Disease of cattle, sheep, pigs, Goats and Horses. 7th edition. Bailliere Tindall, Oxford University Press. |
| [12] | Bordoloi G, Jas R, Ghosh JD (2012) Changes in the haemato-biochemical pattern due to experimentally induced haemonchosis in Sahabadi sheep. J Parasit Dis 36 (1): 101–105. |
| [13] | Bown MD, Poppi DP, Sykes AR (1991) Nitrogen transactions along the digestive tract of lambs concurrently infected with Trichostrongylus colubriformis and Ostertagia circumcincta. Brit J Nutr 66: 237-249. |
| [14] | Busher JT (1990) Serum Albumin and Globulin. In: HK Walker, WD Hall, JW Hurst (Eds.) Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. Boston: Butterworths Publishers. UK. |
| [15] | Cardia DFF, Rocha-Oliveira RA, Tsunemi MH, Amarante AFT (2011) Immune response and performance of growing Santa Ines lambs to artificial Trichostrongylus colubriformis infections. Vet Parasitol 182 (2-4): 248-258. |
| [16] | Chothani DL, Vaghasiya HU (2011) A review on Balanites aegyptiaca Del (desert date): phytochemical constituents traditional uses and pharmacological activity. Pharmacognosy Reviews 5 (9): 55–62. |
| [17] | Doumas BT, Waston WA, Bigges HS (1971) Albumin standards and the measurement of serum albumin with bromocresol green. Clinical Chemistry 31: 87–96. |
| [18] | Dutta B, Konch P, Rahman T et al. (2017) Occurrence and pathology of Haemonchus contortus infection in Goats. Journal of Entomology and Zoology Studies JEZS 5 (3): 1284–1287. |
| [19] | El-Masry SA, Ebeed MM, El-Sayed IH et al. (2010) Protective Effect of Balanites aegyptiaca on Antioxidant Defense System Against Adriamycin-Induced Cardiac Toxicity in Experimental Mice. Egyptian Journal of Biochemistry & Molecular Biology 28 (1): 101–116. |
| [20] | Esmaeilnejad B, Tavassoli M, Asri-Rezaei S, Dalir-Naghadeh B (2012) Evaluation of antioxidant status and oxidative stress in sheep naturally infected with Babesia ovis. Vet Parasitol 185 (2): 124-130. |
| [21] | Ezzat SM, Motaal AA, El Awdan SAW (2017) In vitro and In vivo antidiabetic potential of extracts and a furostanol saponin from Balanites aegyptiaca. Pharm Biol 55: 1931-1936. |
| [22] | Fairbanks VF, Klee GG (1987) Biochemical aspects of hematology. In: NW Tietz (ed). Fundamentals of clinical chemistry, 3rd ed. Philadelphia: WB Saunders. pp 789-824. |
| [23] | Fossati P, Prencipe L (1982) Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem 28: 2077–2080. |
| [24] | Fox MT (1993) Pathophysiology of infection with Ostertagia ostertagi in cattle. Vet Parasitol 46: 143-158. |
| [25] | Gindler EM, King JD (1972) Rapid colorimetric determination of calcium in biologic fluid with Methyl thymol blue. American J Clin Pathol 58: 376-382. |
| [26] | Goodwin JF (1970) Determination of serum phosphorus. Clinical chemistry 16: 776 -780. |
| [27] | Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177: 751-766. |
| [28] | Halliwell B, Gutteridge JMC (2007) Free Radicals in Biology and Medicine, 4th ed. Oxford Clarendon Press. |
| [29] | Hassan LE, Dahham SS, Saghir SA et al. (2016) Chemo therapeutic potentials of the stem bark of Balanites aegyptiaca (L.) Delile: An antiangiogenic, antitumor and antioxidant agent. BMC Complement Altern Med 16 (396): 1-13. |
| [30] | Hayat CS, Khalid M, Iqbal Z, Akhtar M (1999) Haematological and Biochemical disturbances associated with Toxocara vitulorum infection in buffalo calves. Int J Agric Bio 1 (4): 247-249. |
| [31] | Henary RJ (1964) Clinical Chemistry and Principle Techniques. 1st ed. Harper and Roe, New York. |
| [32] | Hiranyachattada P, Hiranyachattada S, Nualplub S, Pubumpen S, Nontasut P (2000) The effects of Trichinella spiralis infection on renal function in rats. Southeast Asian J Trop Med Public Health 31 (3): 463-466. |
| [33] | Hosseini SH, Khazraiinia P, Zaeemi M, Nematollahi AA (2012) Comparative study on clinical pathology changes in experimentally infected sheep with active and arrested larvae of Haemonchus contortus. Comp Clin Pathol 21: 321–326. |
| [34] | Houot O (1985) Interpretation of Clinical Laboratory Tests (Editorss: G Siest, J Henny, F Schiele, DS Young), Biochemical publications: 220-234. |
| [35] | Hunter AR, Mackenzie G (1982) The pathogenesis of a single challenge dose of Haemonchus contortus in lambs under six months of age. Journal of Helminthology 56 (1): 135–144. |
| [36] | Intisar AMO, Goreish I, Shaddad SA, Elamin TH, Eltayeb IB (2013) Anthelmintic activity of Balanites aegyptiaca against Haemoncus contortus in goats. Journal of Pharmaceutical and Biomedical Sciences 30 (30): 1065-1070. |
| [37] | Jaheed E, Mohamed AH, Hassan NMF, Mahran KMA, Nasr SM, Abou-Zeina HAA (2019) Evaluation of the curative effect of Balanites aegyptiaca fruits ethanolic extract on Haemonchosis experimentally induced in Egyptian Baladi goats: Phytoanalytical, parasitological and hematological studies. J Parasitic Dis [online] Available at: https://doi.org/10.1007/s12639-019-01143-1 |
| [38] | Kazmierczak SC, Robertson AF, Catron PA, Briley KP, Kreamer BL, Gourley GR (2002) Direct Spectrophotometric Method for Measurement of Bilirubin in Newborns: Comparasion with HPLC and an Automated Diazo Method. Clinical Chemistry 48 (7): 1096- 97. |
| [39] | Khan MA, Roohi N, Rana MAA (2015) Strongylosis in equines. J Anim Plant Sci 25 (1): 1-9. |
| [40] | Koko WS, Galal M, Khalid HS (2000) Fasciolicidal efficacy of Albizia anthelmintica and Balanites aegyptiaca compared with albendazole. Journal of Ethnopharmacology 71: 247-252. |
| [41] | Kolodziejczyk L, Siemieniuk E, Skrzydlewska E (2005) Antioxidant potential of rat liver in experimental infection with Fasciola hepatica. Parasitol Res 96: 367–372. |
| [42] | Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V (2001) Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 54: 356–361. |
| [43] | Kozat S, Yüksek N, Göz Y, Keleş I (2006) Serum iron, total iron-binding capacity, unbound iron-binding capacity, transferrin saturation, serum copper, and hematological parameters in pregnant Akkaraman ewes infected with gastro-intestinal parasites. Turk J Vet Anim Sci 30: 601-604. |
| [44] | Liu SJ, Liao ZX, Tang ZS et al. (2017) Phytochemicals and biological activities of Artemisia sieversiana. Phytochem Rev 16: 441-460. |
| [45] | Machado V, Da Silva AS, Schafer AS et al. (2014) Relationship between oxidative stress and pathological findings in abomasum of infected lambs by Haemonchus contortus. Pathol Res Pract 210 (12): 812-817. |
| [46] | Mir RA, Chishti MA, Zargar H, Ganie SA (2007) Clinocopathological changes in sheep experimentally infected with Haemonchus contortus. World J Agric Sci 3: 562-566. |
| [47] | Molina E, González-Redondo P, Moreno-Rojas R et al. (2018) Evaluation of haematological, serum biochemical and histopathological parameters of growing rabbits fed Amaranthus dubius. J Anim Physiol Anim Nutr 102: e525–e533. |
| [48] | Murray RK, Granner DK, Mayes PA, Rodwell VW (1990) Harper’s Biochemistry, 22nd ed. Prentice Hall, East Norwalk, CT. |
| [49] | Ortolani EL, Leal MLR, Minervino AHH et al. (2013) Effects of parasitism on cellular immune response in sheep experimentally infected with Haemonchus contortus. Vet Parasitol 196 (1–2): 230–234. |
| [50] | Patton CJ, Crouch SR (1977) Calorimetric determination of urea. Analytical Chemistry 49: 464-469. |
| [51] | Pivoto FL, Torbitz VD, Aires AR et al. (2015) Oxidative stress by Haemonchus contortus in lambs: Influence of treatment with zinc edetate. Res Vet Sci 102: 22-24. |
| [52] | Prophet EB, Mills B, Arrington JB, Sobin LH (1994) Laboratory Methods in Histotecnology. Washington DC: American Registry of Pathology. |
| [53] | Qamar MF, Maqbool A (2012) Biochemical studies and serodiagnosis of haemonchosis in sheep and goats. J Anim Plant Sci 22 (1): 32-38. |
| [54] | Radostits OM, Gay CC, Hinchcliff KW, Constable PD (2007) Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats (Radostits, Veterinary Medicine) 10th Edition, Saunders, USA. |
| [55] | Rashid S (2016) Biochemical and pathological studies on the gastrointestinal nematode- Haemonchus Contortus. PhD Thesis, Departement of Zoology, Aligarh Muslim Univerity, Aligarh, India. |
| [56] | Reitman S, Frankel S (1957) Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28: 56 – 63. |
| [57] | Roberts FHS, O’Sullivan PJ (1950) Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle. Australian Journal of Agricultural Research 1 (1): 99–102. |
| [58] | Schoenfeld RG, Lewellen CJ (1964) A colorimetric method for determination of serum chloride. Clin Chem 10 (6): 533-539. |
| [59] | Sharma DK, Chauhan PPS, Agarwal RD (2001) Changes in the levels of serum enzymes and total protein during experimental haemonchosis in Barbari goats. Small Rumin Res 42: 119–123. |
| [60] | Sharma MC and Joshi C (2002) Serum Mineral and Haematobiochemical Profile of Microfilariae Infected Cattle in India: Its Effects on Production and Therapy. Asian –Australas J Anim Sci 15 (3): 357-365. |
| [61] | Snedecor GW, Cochran WG (1982) Statistical methods, 8th ed. Iowa State University USA. |
| [62] | Soulsby EJL (1982) Textbook of Veterinary Clinical Parasitology, 7th ed. Blackwell Scientific Publications Ltd 24-25 Broad Street Oxford UK: 231-238. |
| [63] | Szasz G (1969) A kinetic photometric method for serum γ-glutamyl transpeptidase. Clin Chem 15 (2): 124-136. |
| [64] | Tehrani A, Javanbakht J, Jani M et al. (2012) Histopathological Study of Haemonchus contortus in Herrik Sheep Abomasum. Journal of Bacteriology & Parasitology 03 (05): 1–5. |
| [65] | Trinder P (1951) A Rapid Method for the Determination of Sodium in Serum. Analyst 76 (907): 596-99. |
| [66] | Wang L, Weller CL (2006) Recent advances in extraction of nutraceuticals from plants. Trends in Food Science and Technology 17 (6): 300–312. |
| [67] | Wildblood LA, Kerr K, Clark OA, Cameron A, Turner OG (2005) Production of eosinophil chemoattractant activity by ovine gastrointestinal nematodes. Veterinary Immunology and lmmunopathology 107: 57- 65. |
| [68] | Yadav JP, Panghal M (2010) Balanites aegyptiaca (L.) Del. (Hingot): A review of its traditional uses, phytochemistry and pharmacological properties. International Journal of Green Pharmacy 4 (3): 140. |
APA Style
Ezatullah Jaheed. (2021). Study of Blood Serum Biochemical Profile and Pathological Changes in Haemonchosis Experimentally Induced in Goats. American Journal of BioScience, 9(3), 95-104. https://doi.org/10.11648/j.ajbio.20210903.14
ACS Style
Ezatullah Jaheed. Study of Blood Serum Biochemical Profile and Pathological Changes in Haemonchosis Experimentally Induced in Goats. Am. J. BioScience 2021, 9(3), 95-104. doi: 10.11648/j.ajbio.20210903.14
AMA Style
Ezatullah Jaheed. Study of Blood Serum Biochemical Profile and Pathological Changes in Haemonchosis Experimentally Induced in Goats. Am J BioScience. 2021;9(3):95-104. doi: 10.11648/j.ajbio.20210903.14
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Download@article{10.11648/j.ajbio.20210903.14,
author = {Ezatullah Jaheed},
title = {Study of Blood Serum Biochemical Profile and Pathological Changes in Haemonchosis Experimentally Induced in Goats},
journal = {American Journal of BioScience},
volume = {9},
number = {3},
pages = {95-104},
doi = {10.11648/j.ajbio.20210903.14},
url = {https://doi.org/10.11648/j.ajbio.20210903.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20210903.14},
abstract = {Haemonchus contortus (H. contortus) parasite causes various functional derangements in the host body. This experimental study was established to appraise the remedial effect of Balanites aegyptiaca fruit’s ethanolic extract (BAF-EE) on experimentally induced haemonchosis in goats. Blood serum biochemical parameters and pathological changes were perused. Twelve male goats were randomly divided into 4 equal groups, G1 (infected-untreated), G2 (infected- BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Experimental infection was conducted with a single oral dose of 10x3 infective third larval stage (L3) of H. contortus at 0-time, whereas treatment with BAF-EE and albendazole was given at a single oral dose of 9g and 5mg/kg body wieght respectively in the 5th week post infection (PI). Blood samples were drawn on zero time, 3rd week PI and then biweekly till the 9th week for serum biochemical analyses. At the end of the experiment, all animals were slaughtered to estimate histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum protein (TP), albumin, albumin/globulin (A/G) ratio, total antioxidant capacity (TAC) and calcium (Ca) levels compared to uninfected goats. Significant elevation was observed in values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (γGT), cholesterol, urea and creatinine of infected animals, while no significant differences were recorded in levels of inorganic phosphorus (P), potassium (K), sodium (Na), chloride (Cl) and total iron binding capacity (TIBC). Histopathological studies conducted on abomasa of infected-untreated animals revealed inflammation, necrosis, petechial hemorrhages and intense cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Histopathological alterations in tissues of infected – treated animals were lower in intensity than those of infected-untreated goats. Results of the present experiment demonstrated that BAF-EE has an anti-parasitic and hepatoprotective effects as well as it improves serum biochemical alterations and oxidative stress in goats infected with H. contortus parasite. The present findings nominate the use of this plant as a novel safe natural anthelmintic agent.},
year = {2021}
}
TY - JOUR T1 - Study of Blood Serum Biochemical Profile and Pathological Changes in Haemonchosis Experimentally Induced in Goats AU - Ezatullah Jaheed Y1 - 2021/06/15 PY - 2021 N1 - https://doi.org/10.11648/j.ajbio.20210903.14 DO - 10.11648/j.ajbio.20210903.14 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 95 EP - 104 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20210903.14 AB - Haemonchus contortus (H. contortus) parasite causes various functional derangements in the host body. This experimental study was established to appraise the remedial effect of Balanites aegyptiaca fruit’s ethanolic extract (BAF-EE) on experimentally induced haemonchosis in goats. Blood serum biochemical parameters and pathological changes were perused. Twelve male goats were randomly divided into 4 equal groups, G1 (infected-untreated), G2 (infected- BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Experimental infection was conducted with a single oral dose of 10x3 infective third larval stage (L3) of H. contortus at 0-time, whereas treatment with BAF-EE and albendazole was given at a single oral dose of 9g and 5mg/kg body wieght respectively in the 5th week post infection (PI). Blood samples were drawn on zero time, 3rd week PI and then biweekly till the 9th week for serum biochemical analyses. At the end of the experiment, all animals were slaughtered to estimate histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum protein (TP), albumin, albumin/globulin (A/G) ratio, total antioxidant capacity (TAC) and calcium (Ca) levels compared to uninfected goats. Significant elevation was observed in values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (γGT), cholesterol, urea and creatinine of infected animals, while no significant differences were recorded in levels of inorganic phosphorus (P), potassium (K), sodium (Na), chloride (Cl) and total iron binding capacity (TIBC). Histopathological studies conducted on abomasa of infected-untreated animals revealed inflammation, necrosis, petechial hemorrhages and intense cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Histopathological alterations in tissues of infected – treated animals were lower in intensity than those of infected-untreated goats. Results of the present experiment demonstrated that BAF-EE has an anti-parasitic and hepatoprotective effects as well as it improves serum biochemical alterations and oxidative stress in goats infected with H. contortus parasite. The present findings nominate the use of this plant as a novel safe natural anthelmintic agent. VL - 9 IS - 3 ER -
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