In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of <i>Distemonanthus benthamianus</i> (Caesalpiniaceae) on Wistars Rats

Ouattara-Soro Fatou Shcherazade; Kouadio Kouakou John; Yao Konan Bertin; Thanon Mariam; Abizi Georges

doi:doi:10.11648/j.ajbio.20210902.14

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In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats

Ouattara-Soro Fatou Shcherazade, Kouadio Kouakou John, Yao Konan Bertin, Thanon Mariam, Abizi Georges

Published in American Journal of BioScience (Volume 9, Issue 2)

Received: 4 March 2021 Accepted: 16 March 2021 Published: 7 April 2021

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Abstract

Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.

Published in	American Journal of BioScience (Volume 9, Issue 2)
DOI	10.11648/j.ajbio.20210902.14
Page(s)	55-62
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antioxidant, Distemonanthus benthamianus, Anti-arthritic

References

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Ouattara-Soro Fatou Shcherazade, Kouadio Kouakou John, Yao Konan Bertin, Thanon Mariam, Abizi Georges. (2021). In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats. American Journal of BioScience, 9(2), 55-62. https://doi.org/10.11648/j.ajbio.20210902.14

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Ouattara-Soro Fatou Shcherazade; Kouadio Kouakou John; Yao Konan Bertin; Thanon Mariam; Abizi Georges. In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats. Am. J. BioScience 2021, 9(2), 55-62. doi: 10.11648/j.ajbio.20210902.14

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AMA Style

Ouattara-Soro Fatou Shcherazade, Kouadio Kouakou John, Yao Konan Bertin, Thanon Mariam, Abizi Georges. In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats. Am J BioScience. 2021;9(2):55-62. doi: 10.11648/j.ajbio.20210902.14

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@article{10.11648/j.ajbio.20210902.14,
  author = {Ouattara-Soro Fatou Shcherazade and Kouadio Kouakou John and Yao Konan Bertin and Thanon Mariam and Abizi Georges},
  title = {In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats},
  journal = {American Journal of BioScience},
  volume = {9},
  number = {2},
  pages = {55-62},
  doi = {10.11648/j.ajbio.20210902.14},
  url = {https://doi.org/10.11648/j.ajbio.20210902.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20210902.14},
  abstract = {Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.},
 year = {2021}
}

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TY  - JOUR
T1  - In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats
AU  - Ouattara-Soro Fatou Shcherazade
AU  - Kouadio Kouakou John
AU  - Yao Konan Bertin
AU  - Thanon Mariam
AU  - Abizi Georges
Y1  - 2021/04/07
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbio.20210902.14
DO  - 10.11648/j.ajbio.20210902.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 55
EP  - 62
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20210902.14
AB  - Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.
VL  - 9
IS  - 2
ER  -

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Author Information

Ouattara-Soro Fatou Shcherazade

Laboratory of Biology and Health, Department of Biosciences, University Felix Houphou?t-Boigny, Abidjan, C?te d’Ivoire
Kouadio Kouakou John

Laboratory of Biology and Health, Department of Biosciences, University Felix Houphou?t-Boigny, Abidjan, C?te d’Ivoire
Yao Konan Bertin

Laboratory of Biology and Health, Department of Biosciences, University Felix Houphou?t-Boigny, Abidjan, C?te d’Ivoire
Thanon Mariam

Laboratory of Biology and Health, Department of Biosciences, University Felix Houphou?t-Boigny, Abidjan, C?te d’Ivoire
Abizi Georges

Laboratory of Biology and Health, Department of Biosciences, University Felix Houphou?t-Boigny, Abidjan, C?te d’Ivoire

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Ouattara-Soro Fatou Shcherazade, Kouadio Kouakou John, Yao Konan Bertin, Thanon Mariam, Abizi Georges. (2021). In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats. American Journal of BioScience, 9(2), 55-62. https://doi.org/10.11648/j.ajbio.20210902.14

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ACS Style

Ouattara-Soro Fatou Shcherazade; Kouadio Kouakou John; Yao Konan Bertin; Thanon Mariam; Abizi Georges. In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats. Am. J. BioScience 2021, 9(2), 55-62. doi: 10.11648/j.ajbio.20210902.14

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AMA Style

Ouattara-Soro Fatou Shcherazade, Kouadio Kouakou John, Yao Konan Bertin, Thanon Mariam, Abizi Georges. In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats. Am J BioScience. 2021;9(2):55-62. doi: 10.11648/j.ajbio.20210902.14

Copy | Download

@article{10.11648/j.ajbio.20210902.14,
  author = {Ouattara-Soro Fatou Shcherazade and Kouadio Kouakou John and Yao Konan Bertin and Thanon Mariam and Abizi Georges},
  title = {In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats},
  journal = {American Journal of BioScience},
  volume = {9},
  number = {2},
  pages = {55-62},
  doi = {10.11648/j.ajbio.20210902.14},
  url = {https://doi.org/10.11648/j.ajbio.20210902.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20210902.14},
  abstract = {Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.},
 year = {2021}
}

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TY  - JOUR
T1  - In Vitro Antioxidant and Anti-arthritic Activity of the Aqueous Extract of the Bark of Distemonanthus benthamianus (Caesalpiniaceae) on Wistars Rats
AU  - Ouattara-Soro Fatou Shcherazade
AU  - Kouadio Kouakou John
AU  - Yao Konan Bertin
AU  - Thanon Mariam
AU  - Abizi Georges
Y1  - 2021/04/07
PY  - 2021
N1  - https://doi.org/10.11648/j.ajbio.20210902.14
DO  - 10.11648/j.ajbio.20210902.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 55
EP  - 62
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20210902.14
AB  - Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.
VL  - 9
IS  - 2
ER  -

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